Comparison of alcohol shock enrichment and selective enrichment for the isolation ofClostridium difficile
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منابع مشابه
A selective enrichment broth for the isolation of Clostridium difficile.
The cycloserine, cefoxitin, fructose agar medium (CCF agar) devised by George et al' and the modification of it where egg yolk is replaced by horse blood (Oxoid CCF agar), have been widely and successfully used for the isolation of Clostridium diffiwile from faecal specimens.'-4 For epidemiological and environmental work where only small numbers of organisms may be present, it is likely that a ...
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Discussion: Considering the effect of sample weight on the detection of Salmonella bacteria (Davies et al., 2000), we cannot directly compare the three enrichment media used herein. However, we suggest the use of M1 whenever possible to obtain large amounts of fecal material (10g). Furthermore, the use of direct enrichment of swine feces in SC is discouraged. The coupling of RV to PCR was signi...
متن کاملIsolation of shigellae. V. Comparison of enrichment broths with stools.
Many enteric media are more efficient for the detection of salmonellae than of shigellae. Comparisons of three enrichment broths and three plating media were made during analysis of 1,405 stool specimens to choose a combination of media which would enhance detection of shigellae as well. Gram-Negative (GN), Selenite, and Silliker's Broths were streaked to E M B, Salmonella-Shigella (SS), and xy...
متن کاملSelective enrichment broth culture for detection of Clostridium difficile and associated cytotoxin.
A procedure was devised for routine examination of feces for Clostridium difficile with selective enrichment broth culture containing increased levels of carbohydrates and antibiotics to detect cytotoxin and volatile acids in broths inoculated with fecal samples. C. difficile was detected and identified with a rapidity comparable to that of conventional culture on selective cycloserine-cefoxiti...
متن کاملThe use of two enrichment forms (Brachionus plicatilis enrichment and rearing water enrichment) with probiotic bacilli spore on growth and survival of Silver carp (Hypophthalmichthys molitrix)
This study was carried out to evaluate the effect of probiotic (Bacillus latrospores and Bacillus licheniformis) on growth and survival of Silver carp larvae (Hypophthalmichthys molitrix). Rotifers (Brachionus plicatilis) were used as live prey vehicle for probiotic transport. They were cultured in sea water with 15 ppt salinity and Nannochloropsis oculata microalgae was used as feed. Five trea...
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ژورنال
عنوان ژورنال: Epidemiology and Infection
سال: 1987
ISSN: 0950-2688,1469-4409
DOI: 10.1017/s0950268800067832